Structure and regulation of the human growth hormone-releasing hormone receptor gene.

Comparison of Expression of Growth Hormone-Releasing Hormone and Its Receptor Splice Variant 1 in Different Stages of Endometriosis

The relationship between growth hormone polymorphism and growth hormone receptor genes with milk yield and reproductive performance in Holstein dairy cows

The aim of this study was to investigate the potential association between growth hormone GH/AluI and growth hormone receptor GHR/AluI polymorphisms with milk yield and reproductive performances in Holstein dairy cows in Iran. Blood samples of 150 Holstein cows were collected and their genomic DNA was extracted using Gene-Fanavaran DNA extracting kit. Fragments of the 428 bp of exon 5 growth ho...

Association of TG-repeats in the 5’-flanking region of bovine growth hormone receptor (GHR) gene with milk production traits and somatic cell count in Holstein cattle

The growth hormone receptor (GHR) is a member of cytokine/hematopoietin family that mediates the biological actions of growth hormone (GH) on target tissues. Therefore, the purpose of this study was to examine the association of TG-repeat polymorphisms in the 5’-flanking region of bovine GHR gene with milk production traits and somatic cell score (SCS) in Holstein cattle of Iran. The part of 5’...

P-24: Opioid and Progesterone Signaling Is Obligatoryfor Early Human Embryogenesis

Background: The growth factors that drive the division and differentiation of stem cells during early human embryogenesis are unknown. The secretion of endorphins, progesterone (P(4)), human chorionic gonadotropin, 17beta-estradiol, and gonadotropin-releasing hormone by trophoblasts that lie adjacent to the embryoblast in the blastocyst suggests that these pregnancy-associated factors may direc...

RT-PCR MEDIATED CLONING OF HUMAN GROWTH HORMONE GENE AND I TS EXPRESSION IN E. coli

Human growth hormone (hGH) genomic sequence containing 5 exons and 4 introns was cloned in pcDNA-3 and the constructed plasmid was subsequently used for transfection ofNlli-3T3 cell line using lipofection technique. Expression of hGH in stably transfected cells was assayed using ELISA. Total RNA was extracted from transfected cells and hGH cDNA was amplified by RT-PCR using specific primers...

P-127: The Effect of Beta Globin Intron on Human FSH Hormone Expression in CHO Cells

Background Follicle stimulating hormone (FSH)- a hetrodimeric glycoprotein- is secreted by pituitary gland. This hormone stimulates growth and maturation of the follicles in females and sperms in male. Up to now, glycoprotein hormones such as FSH have produced in different cell lines. Among of the mammalian expression systems, the Chinese hamster ovary cells (CHO) have taken into consideration ...